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Does anyone out there still believe that Pfu Turbo (or Ultra™) is worth it in terms of site-directed mutagenesis? Ricardipus or Hal, any ideas?
Yes, it has been a while since I've done it, but I am certain I used vanilla Pfu last time. Which, for us at least, has certain cost benefits. And I had a go at Hal a couple of months ago on this very subject, it seems.
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Comments
Darned if I know. Didn't we all used to get along fine with 'vanilla' proofreading polymerases?
/grumpy old codger
Posted by: The Whiffler called Ricardipus | October 24, 2006 10:00 AM
As a lowly honours student in a molecular bio lab, I have to say I found Pfu Turbo (and indeed the whole QuickChange kit) an absolute lifesaver.
True, that might have been as much due to the simplicity of the shake-n-bake style instructions (always a blessing to a bewildered student) as the quality of the polymerase. But still, I have a thesis because of it. And therefore worth it. Other would-be honours students take heed!
Posted by: Andrew | October 24, 2006 10:14 AM
Kit? Kit?! Bah. You youngsters don't know you're born (rant brewing).
Seriously, you can do the QuikChange method without ever buying the kit, just as quickly and for less money. It's only PCR and a smidge of Dpn I, after all.
Posted by: BK | October 24, 2006 10:21 AM