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(No, I'm not dead)

We've had our cohort of Honours students give their "nearly finished lab work" progress reports this week. This took the form of ten minute presentations to their peers and the assessment panel in a fairly informal atmosphere. We did not formally assess the students at this stage but offered what I hope were helpful suggestions and probed how well they have been doing.

Despite a bit of low-to-medium grilling they came acquitted themselves admirably. Most of the students have not only done a lot of work but have also achieved something, which is quite remarkable in the relatively short time they've had. I have been meaning to write about my own very young apprentice and how he now runs a cloning masterclass; it looks like he'll be taking over the lab's NMR work too, so successful have been his experiments.

Ten students gave talks, and on the whole I was impressed with the clarity and organization of the presentations. There were a few crowded and unclear slides, but these were notable for being so rare. I am particularly gratified to be able to report not a single instance of Comic Sans.

Naturally, there were one or two blunders, and for those who enjoyed my previous rant on seminar-giving, here's some more advice (in no particular order, just as they occurred to me during the talks):

It is essential to give a good impression, and equally to end on a high note. "Er, that's all I have" is not how to end a talk, and neither should you begin by saying "The title of my project is. . ." when the title of your project is already on the screen for us all to read. FSP wrote about this a month ago: unfortunately I only read her very useful advice fifteen minutes before kick-off this morning and was unable to pass the link around the troops in time. Think seriously about how you're going to introduce your talk, and indeed your introduction. I have to say that the introductions given in the last two days were very good; contextualizing a project and leaving enough time talk about your work when you only have ten minutes is harder than it sounds.

Introductions do not have to be formally written in complete sentences. Bullet points are fine; the shorter and punchier the better. Right-justification of text on slides looks daft, especially when you have         long words      and    big      gaps.

When you show gel-based data, do not splice together lanes from different gels (especially if on one section you have markers and nothing else!). This used to be accepted but journals are becoming increasingly agitated about this — with good reason — and it's a bad habit to get into.

Reading from slides — I've talked about this before, but similarly, you should not read from notes or talk to the screen. It was only ten minutes, and I would have expected every student to have been able to present without notes.

Use the active voice. Do not say "This protein was purified by affinity chromatography . . .", but rather "I purified this protein . . .". You did it; be proud of that. Similarly, "NMR was used to solve the structure . . ." is a little bit insulting to the guy in the lab (and who might be in the audience) who did it. "Dr Chou solved this structure by NMR . . ." is much better. People have names — use them. And for goodness' sake spell them correctly in your acknowledgments.

Learn the multiples of words like 'spectrum' and 'criterion'. Saying 'spectras' is just silly.

Summarize well; reiterate what you've actually done. And make sure your file is on the internal disk of the computer from which you are going to give the presentation, and that it displays correctly, before you start.

All that aside, a 'very well done' to our Honours students. I look forward to reading your theses.

The powercut last night has screwed up the networking for the PoS PC that runs the phosphoimager, so I can't get my data off it.

A communal piece of equipment that I'd like to use is booked this morning from 09:30 to 12:30: at 10:40 it was still idle.

The door-swipy system that protects our offices from pilfering students is powered off so I couldn't get into the office just now. Fortunately the phones still worked.

More...

HeLa cells , n.

hela.jpg

Evil little bastards. I suspect Henrietta wasn't too happy with them, either.

Too much

19 July, 2007

Derek complains that his labs have had too much (redundant) equipment.

Bastard. I wish our lab was so elegantly over-stacked.

Chemicals, on the other hand, oh we have lots of those that have been ordered, used maybe once if at all, and left, forlorn and forgotten, at the bottom of a freezer. And sometimes on the shelf, right next to the marbles:

More...

Out at Black Towers, on the fringes of the Inner West, we have an air conditioning unit.

Today was the first time we've ever used it in anger. On reverse-cycle. There was frost on the grass. Frost! In Sydney!

I'm glad we didn't move here for the weather.

The Black Knight has been hard at party this last week, which accounts for his silence. We had outlaws over from foreign climes, dontcherknow, and far too much fun.

But it's pleasing to see that the Cage carries on in its inimitable way, with the usual angry students and requests for obscure reagents. Gratifyingly, however, at least one Rat's whiskers are twitchy enough to realize that I live for this stuff, and in his email to one of the Cage's lists attempts to forestall me by saying

P.S.: To the black knight: This is not a plan to take over the building / world

As it happens, I'm not so much concerned with Mr Kidd's missing post, as with the MYSTERIOUS MUG THIEF.

Back in May, this email did the rounds:

ok I want you to all stop what you are doing, we have an emergency, we have a missing mug, this is not just any mug this one belongs to a VIP and they would like it returned asap. I will include a detailed description so if you could all check the area of the building where you are it would be appreciated. (A general search of the building may have to follow if this email fails to deliver results).

A VIP? Ooh. The email continued,

The mug is large (very large) red in colour and has the Nescafe logo on it, it was last seen in the tea room from where it went missing, if you find this mug please return it to the tea room and no questions will be asked.

Serious stuff. I did switch off a little bit when I saw the 'N' word, and honestly thought no more of it for two weeks, until this appeared in our mailboxes:

I'm missing my mug! It disappeared after the amoeba trade display last week. It's a conical shape, white, with letters and words in a light brown color on the outside.

Another mug, mysteriously spirited away in the middle of the night, to who-knows-where for who-knows-what, and the intriguing possibility that we had a serial mugnapper in the Cage. (And yeah, saying what the 'letters and words' actually were might have helped).

I think the entire department must have gone to Safe Base Delta, because for over a month there were no further reports of mugs going missing, mysteriously or otherwise. We thought, if indeed we thought about the matter at all, that the mugs must have been returned to their owners (or at least the VIP's; there were no repercussions, and China, for example, was not invaded to secure future consistency of drinking utensil supply).

But it turns out that the mug thief, or thieves, had merely been biding their time and waiting for the heightened security alert to relax. Yesterday:

if you happen to see any of the tea room mugs/cups on your way around the building could you please drop them off in the kitchen, as we seem to be missing rather a lot

What is this? A ring of hardened international criminals, scouring the Cage for lonesome mugs? A Scientologist recruitment drive? What is going on? Will we ever find out?

Tune in next week for the next exciting episode of "Life of a Lab Rat" and find out! Or not.

Exchange during a group meeting:

"So, if you're a yeast cell —"

"What are you doing in our lab meeting?"


More...

deuteragonist , n.

In a structural laboratory, one who labels his samples with 2H.

e.g. "Jill says that to be successful at small angle neutron scattering you have to be a good deuteragonist."

c.f. protagonist

From Nature's blogs, this week:

NMR always seems more elegant and cultured (surely spin choreography trumps reciprocal space) than crystallography

Discuss.

County Fair

4 July, 2007

I got to play on the confocal and multiphoton microscopes yesterday. I was even allowed to drive the multiphoton myself, which has cool green laser tubes and all sorts of funky stuff and is basically more fun than a postdoc should be allowed to have (so don't tell my boss, 'mkay?).

It was basically a driving lesson for me, to figure out what the equipment can do, how to use it, and only secondarily to examine my cells to see if we can figure out what the fruitbat they are doing. So this picture has no scientific merit, but is very pretty:

koala bear

I said to the Younger Pawn (aged seven and a half!) this morning "Come and see a koala bear!" and showed her the picture.

"Koalas aren't bears!" she stroppily informed me.

"Oh, what are they?"

"Marsupials" she said, definitely.

Sigh. Trust kids to take the fun out of science.

About the Rat

Black Knight is interested in the interaction of science (as a day job and as a way of thinking) with his family, the wider community and literature. And tormenting students. Frequently polemical, sometimes serious, and hopefully always entertaining more

blackasknight@gmail.com

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