
How is it that one little gel can make a post-doc so happy?
BOUNCE
(Shame the Westerns are still a bit crap, but they seem to be corroborating what's going on in lanes 3, 9 and 10 of the lower strip).
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How is it that one little gel can make a post-doc so happy?
BOUNCE
(Shame the Westerns are still a bit crap, but they seem to be corroborating what's going on in lanes 3, 9 and 10 of the lower strip).
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Comments
You know, before the jpeg loaded and reading it from an American bias, I thought there would be some picture of you with your fiancee or daughter or something.
Now you have to do it again with qRT-PCR?
Posted by: Alethea | March 27, 2008 06:54 PM
heh heh.
(No; that's what grad students are for. I have to do it with an exon microarray.)
Posted by: bk | March 27, 2008 07:50 PM
I'm just a lowly undergrad, but I still get happy when a gel goes right...simply because of all the times I've had a gel go wrong. I spent last spring semester helping with DGGE gels (and all the fun PCR work from nesting primers), and this summer I get to work on sds-PAGE gels. The success or failure of a gel tends to sit with the lab for the day.
Posted by: Kim | March 28, 2008 04:12 AM
...and I am curious of what the lanes 11 and 12 are controls for?! 11 I can guess maybe for the lower band but 12??
(and no, you don't have to answer, research priveliges and all. Just letting you know my first thoughts after "Oh, I wish my gel could be as clear!")
Posted by: chall | March 28, 2008 07:05 AM
They're cDNA controls. 11 is the coding sequence of the gene we're interested in, 12 the empty (GFP) vector.
I've been keeping the TM low because this PCR was giving me a bit of trouble, which is why there's non-specific bands in the top row.
That lower band appears to be primers/dNTPs.
Posted by: bk | March 28, 2008 09:17 AM
Yay!
"that's what grad students are for" --> =-P
I'm having a bad case of "ugly sds gel syndrome" this week, good do hear someone's experiments are going well!
Posted by: Maxwell's Demoness | March 29, 2008 05:01 AM
I should add that this is the first time since the middle of last year that this experiment has actually worked. *sigh*
Posted by: bk | March 29, 2008 12:55 PM
Um, if you're validating the results by qPCR (in triplicate I'm sure), why bother with the exon array? Nasty, messy, expensive things.
Posted by: Whiffler | March 31, 2008 01:22 AM
Because the exon microarray is kind of the point.
Posted by: bk | March 31, 2008 06:31 AM
thanks. That made my day less confusing... or at least there was an answer which I don't see in my own experiment at the moment ;)
Posted by: chall | March 31, 2008 10:51 AM
Great! Just so you are more careful next time, primer (dimers) is alright, not dNTPs.
Posted by: dimmy | April 5, 2008 08:13 AM