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Sweetest thing

30 April, 2008

westerned.jpg

It might not look much to you, but that rather messy blot up there helped to tell me three things this afternoon:

  1. The original antibody is crap and does not reliably and uniquely detect my protein. I suspected this already
  2. The detection reagents give a better signal to noise ratio when you leave them for over an hour before exposing to film (data not shown. Hopefully I'll get a nicer picture of that tomorrow)
  3. The knockouts are working again.

It worked. It bloody well worked!

Comments

Of course, I have no idea what it really MEANS but I do love that image. Can I have it for my wall?

Seriously?

I'll re-scan at higher res if you like.

Yep. You could send it through the big file transfer thingo the uni has. If you know what I mean. Otherwise, let me know .

hm, I turn my back for a few seconds and a photo and someone can get it ? ;)

I wouldn't mind it either... but then I kind of like strange things :)

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About the Rat

Black Knight is interested in the interaction of science (as a day job and as a way of thinking) with his family, the wider community and literature. And tormenting students. Frequently polemical, sometimes serious, and hopefully always entertaining more

blackasknight@gmail.com

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