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Spooling up

4 August, 2008

After a long (too bloody long) time geeking around with perl, preparing posters and talks, and doing some hardcore microarray data mangling, I went into the lab this morning and filled my ice bucket. I got out my cut vectors and inserts and found the water and ligase buffer. It's all on ice, thawing, so for the first time in about six weeks I'm going to do a real experiment (keeping cells alive doesn't count).

It feels good. I'm all set. I'm ready, and now I'm going...

to have a cup of coffee. Yeah.

Comments

Oh, god. Does that mean you've already prepared your talk for England at the end of the month? I'll have to kill you if so. I'm drowning, here.

oh heck, no.

I thought I'd just wing that. Seems to be working for the rest of my life.

I'm relieved. How did your ligation come out? Do your miniprep today?

cloning experiment from hell. Colony PCR.

Yeesh.

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About the Rat

Black Knight is interested in the interaction of science (as a day job and as a way of thinking) with his family, the wider community and literature. And tormenting students. Frequently polemical, sometimes serious, and hopefully always entertaining more

blackasknight@gmail.com

Recent Comments

  • bk said "cloning experiment from hell. Colony PCR. Yeesh."
  • Alethea said "I'm relieved. How did your ligation come out? Do y"
  • bk said "oh heck, no. I thought I'd just wing that. Seem"
  • Alethea said "Oh, god. Does that mean you've already prepared yo"

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