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So... there is something absorbing at 270 nm in E1, and it's unlikely to be protein because there's only a minimal peptide bond peak at 230 nm. E2 and E3 look fine, absorbing at 230 and 280 nm.
This kind of weirdness happens on a more or less daily basis in the lab.
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Comments
Phenol.
Posted by: Ed Rybicki | October 31, 2008 12:03 AM
Well, you'd think so: but there was no phenol near that prep.
Posted by: bk | October 31, 2008 09:03 AM